241-9Genetic Diversity Among Soybean Genotypes Selected for Hybridizations and Directed for Rotation with Sugar Cane.
See more from this Division: C01 Crop Breeding & GeneticsSee more from this Session: Use of Molecular Tools to Enhance Breeding Efforts
Tuesday, October 23, 2012
Duke Energy Convention Center, Exhibit Hall AB, Level 1
Soybean has assumed increasing importance in sugarcane areas of São Paulo State (Brazil) as an option for the cultivation of rotation with sugar cane. However, it is necessary that the cultivation has a short life cycle to meet the schedule of sugar cane, as well as good agronomic traits. Thus, we seek to obtain promising soybean genotypes for sugarcane rotation system through artificial crossings between parental carries earliness, yield grain, disease resistance and high oil content. Within this focus the objective of this study was to evaluate the promising parental with genetic diversity for these characters, allowing the identification of the most contrasting parental for artificial hybridization, resulting in greater genetic variability. We used 57 Simple Sequence Repeat (SSR) markers to assess the genetic diversity of 23 soybean genotypes. The SSR markers were assessed using 3% (w/v) agarose gels stained with ethidium bromide and genotyped considering the presence or absence of bands. The results of molecular analyzes provides a standard of 135 polymorphic bands that were subjected to clustering analysis using Jaccard similarity coefficients and the Unweighted Pair Group Method with Arithmetic mean (UPGMA). The constructed dendrogram presented a maximum Jaccard index of 0.66, indicating a moderate genetic diversity among the evaluated genotypes. However, there was formation of 13 distinct groups, being L1 and Orba, the most genotypes divergent. Through this analysis will be possible to direct the crossings to obtain a greater genetic variability between the selected promising parental for the desired attributes.
See more from this Division: C01 Crop Breeding & GeneticsSee more from this Session: Use of Molecular Tools to Enhance Breeding Efforts