95-24Multi-Environment Quantitative Trait Loci Analysis for Resistance to Stripe Rust and Cephalosporium Stripe in Two Recombinant Inbred Line Populations.
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Monday, October 22, 2012
	Duke Energy Convention Center, Exhibit Hall AB, Level 1
		˙ūS t r i p e   r u s t   ( P u c c i n i a   s t r i i f o r m i s )   a n d   C e p h a l o s p o r i u m   s t r i p e   ( C e p h a l o s p o r i u m   g r a m i n e u m )   c a n   c a u s e   s e v e r e   y i e l d   a n d   g r a i n   q u a l i t y   l o s s   o f   w h e a t   ( T r i t i c u m   a e s t i v u m   L . )   i n   t h e   P a c i f i c   N o r t h w e s t ,   U S A .   F a v o r a b l e   w e a t h e r   c o n d i t i o n s   a n d   t h e   a p p e a r a n c e   o f   n e w   r a c e s   a r e   b e c o m i n g   a n   i m p o r t a n t   f a c t o r   f o r   a n n u a l   d e v e l o p m e n t   o f   s t r i p e   r u s t   d i s e a s e   i n   t h e   r e g i o n .   C e p h a l o s p o r i u m   s t r i p e   c a n   b e   a   l i m i t i n g   f a c t o r   i n   t h e   a d o p t i o n   o f   c o n s e r v a t i o n   t i l l a g e   p r a c t i c e s   a n d   l i t t l e   i s   k n o w n   a b o u t   t h e   i n h e r i t a n c e   o f   r e s i s t a n c e .   T w o   r e c o m b i n a n t   i n b r e d   l i n e s   ( R I L s )   p o p u l a t i o n s   ( E i n s t e i n   x   T u b b s   a n d   T u b b s   x   N S A   9 5 - 0 9 9 5 )   w e r e   a s s e s s e d   f o r   q u a n t i t a t i v e   t r a i t   l o c i   ( Q T L )   a n a l y s i s   t o   s t r i p e   r u s t   a n d   C e p h a l o s p o r i u m   s t r i p e .   A   l i n k a g e   m a p   w a s   c r e a t e d   f o r   e a c h   p o p u l a t i o n   b a s e d   o n   d i v e r s i t y   a r r a y   t e c h n o l o g y   ( D A r T )   a n d   s i m p l e   s e q u e n c e   r e p e a t   ( S S R )   m a r k e r s .   F i e l d   s c r e e n i n g s   f o r   s t r i p e   r u s t   o n   b o t h   p o p u l a t i o n s   w e r e   d o n e   i n   s i x   e n v i r o n m e n t s   u n d e r   n a t u r a l   i n o c u l a t i o n .   F i e l d   s c r e e n i n g s   f o r   C e p h a l o s p o r i u m   s t r i p e   u n d e r   a r t i f i c i a l l y   i n o c u l a t e d   c o n d i t i o n s   a n d   b a s e d   o n   w h i t e h e a d s   ( s t e r i l e   h e a d s   c a u s e d   b y   p a t h o g e n   i n f e c t i o n )   w e r e   d o n e   i n   f o u r   e n v i r o n m e n t s   f o r   t h e   E i n s t e i n   x   T u b b s   p o p u l a t i o n   a n d   i n   t h r e e   e n v i r o n m e n t s   f o r   t h e   T u b b s   x   N S A   9 5 - 0 9 9 5   p o p u l a t i o n .   R e s u l t s   f r o m   t h e   Q T L   a n a l y s i s   i d e n t i f i e d   c o m m o n   r e g i o n s   a s s o c i a t e d   w i t h   r e s i s t a n c e   t o   s t r i p e   r u s t   a n d   C e p h a l o s p o r i u m   s t r i p e .   I n   a d d i t i o n ,   a s o c i a t i o n   m a p p i n g   i n   6 4   l i n e s   f r o m   t h e   P a c i f i c   N o r t h w e s t   U S A   i s   p e r f o r m e d   t o   v a l i d a t e   r e s u l t s   f r o m   t h e   Q T L   a n a l y s i s .   
	
	
	
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